Proteomics-based diagnostic peptide discovery for severe fever with thrombocytopenia syndrome virus in patients.

ABSTRACT:

Background

Severe fever with thrombocytopenia syndrome (SFTS) virus is an emerging infectious virus which causes severe hemorrhage, thrombocytopenia, and leukopenia, with a high fatality rate. Since there is no approved therapeutics or vaccines for SFTS, early diagnosis is essential to manage this infectious disease.

Methods

Here, we tried to detect SFTS virus in serum samples from SFTS patients by proteomic analysis. Firstly, in order to obtain the reference MS/MS spectral data of SFTS virus, medium from infected Vero cell culture was used for shotgun proteomic analysis. Then, tryptic peptides in sera from SFTS patients were confirmed by comparative analysis with the reference MS/MS spectral data of SFTS virus.

Results

Proteomic analysis of culture medium successfull

SUBMITTER: Lee SY 

PROVIDER: S-EPMC9287713 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature