N-terminus of flagellin enhances vaccine efficacy against Actinobacillus pleuropneumoniae.

ABSTRACT:

Background

Flagellin elicits potent immune response and may serve as a vaccine adjuvant. We previously reported that the N-terminus of flagellin (residues 1-99, nFliC) is sufficient for vaccine efficacy enhancement against Pasteurella multocida challenge in chickens. In this study, we futher tested the adjuvancy of nFliC in a subunit vaccine against the pig pathogen Actinobacillus pleuropneumoniae in a mice model. For vaccine formulation, the antigen ApxIIPF (the pore-forming region of the exotoxin ApxII) was combined with nFliC, either through genetic fusion or simple admixture.

Results

Immune analysis showed that nFliC, introduced through genetic fusion or admixture, enhanced both humoral (antibody levels) and cellular (T cell response and cytokine production) immunity. In

SUBMITTER: Chuekwon K 

PROVIDER: S-EPMC9288005 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature