Method for detecting acetylated PD-L1 in cell lysates by immunoprecipitation and western blot analysis.

ABSTRACT: Lysine acetylation is an important regulatory post-translational modification (PTM) that occurs sub-stoichiometrically, often representing less than 1% of the target protein. This makes studying endogenous protein acetylation extremely challenging. Recent reports suggest that several post-translational modifications (PTMs), including lysine acetylation, play a major role in the regulation the programmed cell death-ligand 1 (PD-L1), a clinically important protein target. An enrichment step is necessary to enable identification of the acetylated species by either antibody or mass spectrometry-based detection methods. This report describes a robust lab protocol for the enrichment and detection of endogenous acetylated PD-L1 protein. A recently developed acetyl lysine affinity matrix was utili

SUBMITTER: Middleton-Davis F 

PROVIDER: S-EPMC9292098 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature