Ontology highlight
ABSTRACT:
SUBMITTER: Heynck L
PROVIDER: S-EPMC9297387 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Heynck Lukas L Matthias Jessica J Bossi Mariano L ML Butkevich Alexey N AN Hell Stefan W SW
Chemical science 20220627 28
Fluorescein and its analogues have found only limited use in biological imaging because of the poor photostability and cell membrane impermeability of their <i>O</i>-unprotected forms. Herein, we report rationally designed <i>N</i>-cyanorhodamines as orange- to red-emitting, photostable and cell-permeant fluorescent labels negatively charged at physiological pH values and thus devoid of off-targeting artifacts often observed for cationic fluorophores. In combination with well-established fluores ...[more]