Evidence for tetrodotoxin-resistant spontaneous myogenic contractions of mouse isolated stomach that are dependent on acetylcholine.

ABSTRACT:

Background and purpose

Gastric pacemaker cells, interstitial cells of Cajal (ICC), are believed to initiate myogenic (non-neuronal) contractions. These become damaged in gastroparesis, associated with dysrhythmic electrical activity and nausea. We utilised mouse isolated stomach to model myogenic contractions and investigate their origin and actions of interstitial cells of Cajal modulators.

Experimental approach

Intraluminal pressure was recorded following distension with a physiological volume; tone, contraction amplitude and frequency were quantified. Compounds were bath applied.

Key results

The stomach exhibited regular large amplitude contractions (median amplitude 9.0 [4.7-14.8] cmH₂ O, frequency 2.9 [2.5-3.4] c.p.m; n = 20), appearing to progress abo

SUBMITTER: Cai W 

PROVIDER: S-EPMC9297954 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature