Nuclear chromosome locations dictate segregation error frequencies.

ABSTRACT: Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis^1-5. Selective pressures then shape distinct aneuploidy and rearrangement patterns-for example, in cancer^6,7-but it is unknown whether initial biases in segregation errors and micronucleation exist for particular chromosomes. Using single-cell DNA sequencing⁸ after an error-prone mitosis in untransformed, diploid cell lines and organoids, we show that chromosomes have different segregation error frequencies that result in non-random aneuploidy landscapes. Isolation and sequencing of single micronuclei from these cells showed that mis-segregating chromosomes frequently also preferentially beco

SUBMITTER: Klaasen SJ 

PROVIDER: S-EPMC9300461 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature