Degradable Polyampholytes from Radical Ring-Opening Copolymerization Enhance Cellular Cryopreservation.

ABSTRACT: Macromolecular cryoprotectants based on polyampholytes are showing promise as supplemental cryoprotectants alongside conventional DMSO-based freezing. Here we exploit radical ring-opening (ter)polymerization to access ester-containing cryoprotective polyampholytes, which were shown to be degradable. Using a challenging cell monolayer cryopreservation model, the degradable polyampholytes were found to enhance post-thaw recovery when supplemented into DMSO. This demonstrates that degradable macromolecular cryoprotectants can be developed for application in biotechnology and biomedicine.

SUBMITTER: Pesenti T 

PROVIDER: S-EPMC9301905 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature