An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates.

ABSTRACT: We present a low-cost, do-it-yourself system for complex mammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrate the generation of complex concentration profiles, enabling the investigation of sophisticated input-response relations. We further apply our automated cell-culturing platform to the dynamic stimulation of two widely employed stem-cell-based in vitro models for early mammalian development: the conversion of naive mouse embryonic stem cells into epiblast-like cells and mouse 3D gastruloids. Performing automated medium-switch experiments, we systematically investigate cell fate commitment along the developmental trajectory toward m

SUBMITTER: Tischler J 

PROVIDER: S-EPMC9308133 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature