MS-Driven Metabolic Alterations Are Recapitulated in iPSC-Derived Astrocytes.

ABSTRACT:

Objective

Astrocytes play a significant role in the pathology of multiple sclerosis (MS). Nevertheless, for ethical reasons, most studies in these cells were performed using the Experimental Autoimmune Encephalomyelitis model. As there are significant differences between human and mouse cells, we aimed here to better characterize astrocytes from patients with MS (PwMS), focusing mainly on mitochondrial function and cell metabolism.

Methods

We obtained and characterized induced pluripotent stem cell (iPSC)-derived astrocytes from three PwMS and three unaffected controls, and performed electron microscopy, flow cytometry, cytokine and glutamate measurements, gene expression, in situ respiration, and metabolomics. We validated our findings using a single-nuclei RNA sequencing d

SUBMITTER: Ghirotto B 

PROVIDER: S-EPMC9310856 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature