In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi-species biofilm community.

ABSTRACT:

Aim

To evaluate the physicochemical properties of five root canal sealers and assess their effect on an ex vivo dental plaque-derived polymicrobial community.

Methodology

Dental plaque-derived microbial communities were exposed to the sealers (AH Plus [AHP], GuttaFlow Bioseal [GFB], Endoseal MTA [ESM], Bio-C sealer [BCS] and BioRoot RCS [BRR]) for 3, 6 and 18 h. The sealers' effect on the biofilm biomass and metabolic activity was quantified using crystal violet (CV) staining and MTT assay, respectively. Biofilm community composition and morphology were assessed by denaturing gradient gel electrophoresis (DGGE), 16S rRNA sequencing and scanning electron microscopy. The ISO6876:2012 specifications were followed to determine the setting time, radiopacity, flowability and solub

SUBMITTER: Saavedra FM 

PROVIDER: S-EPMC9321831 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature