Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System.

ABSTRACT:

Background

The cancer cell fusion theory could be one of the best explanations for the metastasis from primary tumours.

Methods

Herein, we co-cultured colorectal cancer (CRC) stem cells with human monocytes and analysed the properties of the generated tumour hybrid cells (THCs). The presence of THCs in the bloodstream together with samples from primary and metastatic lesions and their clinical correlations were evaluated in CRC patients and were detected by both FACS and immunofluorescence methods. Additionally, the role of SIGLEC5 as an immune evasion molecule in colorectal cancer was evaluated.

Results

Our data demonstrated the generation of THCs after the in vitro co-culture of CRC stem cells and monocytes. These cells, defined as CD45⁺CD14⁺Ep

SUBMITTER: Montalban-Hernandez K 

PROVIDER: S-EPMC9324286 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature