A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.

ABSTRACT: Dual-color single-molecule localization microscopy (SMLM) provides unprecedented possibilities for detailed studies of colocalization of different molecular species in a cell. However, the informational richness of the data is not fully exploited by current analysis tools that often reduce colocalization to a single value. Here, we describe a tool specifically designed for determination of co-localization in both 2D and 3D from SMLM data. The approach uses a function that describes the relative enrichment of one molecular species on the density distribution of a reference species. The function reframes the question of colocalization by providing a density-context relevant to multiple biological questions. Moreover, the function visualize enrichment (i.e. colocalization) directly in the ima

SUBMITTER: Ejdrup AL 

PROVIDER: S-EPMC9334352 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature