DNA Bending Force Facilitates Z-DNA Formation under Physiological Salt Conditions.

ABSTRACT: Z-DNA, a noncanonical helical structure of double-stranded DNA (dsDNA), plays pivotal roles in various biological processes, including transcription regulation. Mechanical stresses on dsDNA, such as twisting and stretching, help to form Z-DNA. However, the effect of DNA bending, one of the most common dsDNA deformations, on Z-DNA formation is utterly unknown. Here, we show that DNA bending induces the formation of Z-DNA, that is, more Z-DNA is formed as the bending force becomes stronger. We regulated the bending force on dsDNA by using D-shaped DNA nanostructures. The B-Z transition was observed by single-molecule fluorescence resonance energy transfer. We found that as the bending force became stronger, Z-DNA was formed at lower Mg²⁺ concentrations. When dsDNA contained cytosi

SUBMITTER: Yi J 

PROVIDER: S-EPMC9335521 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature