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Better living through chemistry: CRISPR/Cas engineered T cells for cancer immunotherapy.

ABSTRACT: T cells engineered to express transgenes such as chimeric antigen receptors (CAR) or modified T cell receptors (TCR) represent a new pillar of cancer therapy. Use of CRISPR/Cas gene-editing tools now allows even stronger and more precise control over the fate and function of engineered T cell therapies, including multiplex genome editing to facilitate use of off-the-shelf allogeneic T cells and novel approaches which have the potential to overcome some of the limitations of canonical Cas9-mediated DNA cleavage. This review summarizes the CRISPR/Cas techniques that have been used in preclinical research and outlines those that currently being tested in clinical trials.

SUBMITTER: Wellhausen N

PROVIDER: S-EPMC9337770 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Better living through chemistry: CRISPR/Cas engineered T cells for cancer immunotherapy.

Wellhausen Nils N Agarwal Sangya S Rommel Philipp C PC Gill Saar I SI June Carl H CH

Current opinion in immunology 20211116

T cells engineered to express transgenes such as chimeric antigen receptors (CAR) or modified T cell receptors (TCR) represent a new pillar of cancer therapy. Use of CRISPR/Cas gene-editing tools now allows even stronger and more precise control over the fate and function of engineered T cell therapies, including multiplex genome editing to facilitate use of off-the-shelf allogeneic T cells and novel approaches which have the potential to overcome some of the limitations of canonical Cas9-mediat ...[more]

PMID: 34798542

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