Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation.

ABSTRACT: Spermatogenesis is a complex biological process that produces haploid spermatozoa and requires precise regulation by many tissue-specific factors. In this study, we explored the role and mechanism of Fork head box J2 (FOXJ2, which is highly expressed in spermatocytes) in the regulation of spermatogenesis using a germline-specific conditional Foxj2 knock-in mouse model (Stra8-Cre; Foxj2 ^tg/tg mouse). Foxj2 overexpression in mouse testes led to spermatogenesis failure, which started at the initiation of meiosis, and resulted in male infertility. Lysosomes and autophagy-related genes were upregulated in Stra8-cre; Foxj2 ^tg/tg mouse testes and the number of autolysosomes in the spermatocytes in Stra8-cre; Foxj2 ^tg/tg mice was increased. Chromatin immunoprecipit

SUBMITTER: Bai FR 

PROVIDER: S-EPMC9338950 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature