Dataset Information

The Identity of Human Tissue-Emigrant CD8⁺ T Cells.

ABSTRACT: Lymphocyte migration is essential for adaptive immune surveillance. However, our current understanding of this process is rudimentary, because most human studies have been restricted to immunological analyses of blood and various tissues. To address this knowledge gap, we used an integrated approach to characterize tissue-emigrant lineages in thoracic duct lymph (TDL). The most prevalent immune cells in human and non-human primate efferent lymph were T cells. Cytolytic CD8⁺ T cell subsets with effector-like epigenetic and transcriptional signatures were clonotypically skewed and selectively confined to the intravascular circulation, whereas non-cytolytic CD8⁺ T cell subsets with stem-like epigenetic and transcriptional signatures predominated in tissues and TDL. Moreover, these anatomically distinct gene expression profiles were recapitulated within individual clonotypes, suggesting parallel differentiation programs independent of the expressed antigen receptor. Our collective dataset provides an atlas of the migratory immune system and defines the nature of tissue-emigrant CD8⁺ T cells that recirculate via TDL.

SUBMITTER: Buggert M

PROVIDER: S-EPMC9341432 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

The Identity of Human Tissue-Emigrant CD8<sup>+</sup> T Cells.

Buggert Marcus M Vella Laura A LA Nguyen Son S Wu Vincent H VH Chen Zeyu Z Sekine Takuya T Perez-Potti André A Maldini Colby R CR Manne Sasikanth S Darko Samuel S Ransier Amy A Kuri-Cervantes Leticia L Japp Alberto Sada AS Brody Irene Bukh IB Ivarsson Martin A MA Gorin Jean-Baptiste JB Rivera-Ballesteros Olga O Hertwig Laura L Antel Jack P JP Johnson Matthew E ME Okoye Afam A Picker Louis L Vahedi Golnaz G Sparrelid Ernesto E Llewellyn-Lacey Sian S Gostick Emma E Sandberg Johan K JK Björkström Niklas N Bar-Or Amit A Dori Yoav Y Naji Ali A Canaday David H DH Laufer Terri M TM Wells Andrew D AD Price David A DA Frank Ian I Douek Daniel C DC Wherry E John EJ Itkin Maxim G MG Betts Michael R MR

Cell 20201210 7

Lymphocyte migration is essential for adaptive immune surveillance. However, our current understanding of this process is rudimentary, because most human studies have been restricted to immunological analyses of blood and various tissues. To address this knowledge gap, we used an integrated approach to characterize tissue-emigrant lineages in thoracic duct lymph (TDL). The most prevalent immune cells in human and non-human primate efferent lymph were T cells. Cytolytic CD8<sup>+</sup> T cell sub ...[more]

PMID: 33306960

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Project description:BackgroundTissue-resident CD8+T cells (CD103+CD8+T cells) are the essential effector cell population of anti-tumor immune response in tissue regional immunity. And we have reported that IL-33 can promote the proliferation and effector function of tissue-resident CD103+CD8+T cells. As of now, the immunolocalization and the prognostic values of tissue-resident CD8+T cells in human hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) still remain to be illustrated.MethodsIn our present study, we used the tissue microarrays of HCC and ICC, the multicolor immunohistochemistry (mIHC), and imaging analysis to characterize the tissue-resident CD8+T cells in HCC and ICC tissues. The prognostic values and clinical associations were also analyzed. We also studied the biological functions and the cell-cell communication between tumor-infiltrating CD103+CD8+T cells and other cell types in HCC and ICC based on the published single-cell RNA sequencing (scRNA-seq) data.ResultsOur work unveiled the expressions of CD8 and CD103 and immunolocalization of tissue-resident CD8+T cells in human HCC and ICC. Elevated CD8+T cells indicated a better overall survival (OS) rate, implying that tumor-infiltrating CD8+T cells in HCC and ICC could serve as an independent prognostic factor. Moreover, the number of CD103+CD8+T cells was increased in HCC and ICC tissues compared with adjacent normal tissues. HCC patients defined as CD8highCD103high had a better OS, and the CD8lowCD103low group tended to have a poorer prognosis in ICC. Evaluation of the CD103+CD8+T-cell ratio in CD8+T cells could also be a prognostic predictor for HCC and ICC patients. A higher ratio of CD103+CD8+T cells over total CD8+T cells in HCC tissues was negatively and significantly associated with the advanced pathological stage. The percentage of higher numbers of CD103+CD8+T cells in ICC tissues was negatively and significantly associated with the advanced pathological stage. In contrast, the higher ratio of CD103+CD8+T cells over total CD8+T cells in ICC tissues was negatively and significantly associated with the advanced pathological stage. In addition, single-cell transcriptomics revealed that CD103+CD8+T cells were enriched in genes associated with T-cell activation, proliferation, cytokine function, and T-cell exhaustion.ConclusionThe CD103+ tumor-specific T cells signified an important prognostic marker with improved OS, and the evaluation of the tissue-resident CD103+CD8+T cells might be helpful in assessing the on-treatment response of liver cancer.

Dataset Information

The Identity of Human Tissue-Emigrant CD8⁺ T Cells.

Publications

The Identity of Human Tissue-Emigrant CD8<sup>+</sup> T Cells.

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Dataset Information

The Identity of Human Tissue-Emigrant CD8+ T Cells.

Publications

The Identity of Human Tissue-Emigrant CD8<sup>+</sup> T Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

The Identity of Human Tissue-Emigrant CD8⁺ T Cells.