Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis.

ABSTRACT:

Background

At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous.

Objectives

This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens.

Methods

Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10 mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using

SUBMITTER: Sun C 

PROVIDER: S-EPMC9346521 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature