Propofol enhances stem-like properties of glioma via GABA_AR-dependent Src modulation of ZDHHC5-EZH2 palmitoylation mechanism.

ABSTRACT:

Background

Propofol is a commonly used anesthetic. However, its effects on glioma growth and recurrence remain largely unknown.

Methods

The effect of propofol on glioma growth was demonstrated by a series of in vitro and in vivo experiments (spheroidal formation assay, western blotting, and xenograft model). The acyl-biotin exchange method and liquid chromatography-mass spectrometry assays identified palmitoylation proteins mediated by the domain containing the Asp-His-His-Cys family. Western blotting, co-immunoprecipitation, quantitative real-time polymerase chain reaction, co-immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays were used to explore the mechanisms of the γ-aminobutyric acid receptor (GABA_AR)/Src/ZDHHC5/EZH2 signaling

SUBMITTER: Fan X 

PROVIDER: S-EPMC9351178 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature