Light-driven single-cell rotational adhesion frequency assay.
Ontology highlight
ABSTRACT: Supplementary information
The online version contains supplementary material available at 10.1186/s43593-022-00020-4.
SUBMITTER: Liu Y
PROVIDER: S-EPMC9358104 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Publications
Light-driven single-cell rotational adhesion frequency assay.
Liu Yaoran Y Ding Hongru H Li Jingang J Lou Xin X Yang Mingcheng M Zheng Yuebing Y
eLight 20220808 1
The interaction between cell surface receptors and extracellular ligands is highly related to many physiological processes in living systems. Many techniques have been developed to measure the ligand-receptor binding kinetics at the single-cell level. However, few techniques can measure the physiologically relevant shear binding affinity over a single cell in the clinical environment. Here, we develop a new optical technique, termed single-cell rotational adhesion frequency assay (scRAFA), that ...[more]
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