Measuring anion binding at biomembrane interfaces.

ABSTRACT: The quantification of anion binding by molecular receptors within lipid bilayers remains challenging. Here we measure anion binding in lipid bilayers by creating a fluorescent macrocycle featuring a strong sulfate affinity. We find the determinants of anion binding in lipid bilayers to be different from those expected that govern anion binding in solution. Charge-dense anions H₂PO₄^- and Cl^- that prevail in dimethyl sulfoxide fail to bind to the macrocycle in lipids. In stark contrast, ClO₄^- and I^- that hardly bind in dimethyl sulfoxide show surprisingly significant affinities for the macrocycle in lipids. We reveal a lipid bilayer anion binding principle that depends on anion polarisability and bilayer penetration

SUBMITTER: Wu X 

PROVIDER: S-EPMC9359984 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature