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Measuring anion binding at biomembrane interfaces.

ABSTRACT: The quantification of anion binding by molecular receptors within lipid bilayers remains challenging. Here we measure anion binding in lipid bilayers by creating a fluorescent macrocycle featuring a strong sulfate affinity. We find the determinants of anion binding in lipid bilayers to be different from those expected that govern anion binding in solution. Charge-dense anions H₂PO₄^- and Cl^- that prevail in dimethyl sulfoxide fail to bind to the macrocycle in lipids. In stark contrast, ClO₄^- and I^- that hardly bind in dimethyl sulfoxide show surprisingly significant affinities for the macrocycle in lipids. We reveal a lipid bilayer anion binding principle that depends on anion polarisability and bilayer penetration depth of complexes leading to unexpected advantages of charge-diffuse anions. These insights enhance our understanding of how biological systems select anions and guide the design of functional molecular systems operating at biomembrane interfaces.

SUBMITTER: Wu X

PROVIDER: S-EPMC9359984 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Measuring anion binding at biomembrane interfaces.

Wu Xin X Wang Patrick P Lewis William W Jiang Yun-Bao YB Gale Philip A PA

Nature communications 20220808 1

The quantification of anion binding by molecular receptors within lipid bilayers remains challenging. Here we measure anion binding in lipid bilayers by creating a fluorescent macrocycle featuring a strong sulfate affinity. We find the determinants of anion binding in lipid bilayers to be different from those expected that govern anion binding in solution. Charge-dense anions H2PO4- and Cl- that prevail in dimethyl sulfoxide fail to bind to the macrocy ...[more]

PMID: 35941124

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