In vitro differences in toddalolactone metabolism in various species and its effect on cytochrome P450 expression.

ABSTRACT:

Context

Toddalolactone, the main component of Toddalia asiatica (L.) Lam. (Rutaceae), has anticancer, antihypertension, anti-inflammatory, and antifungal activities.

Objective

This study investigated the metabolic characteristics of toddalolactone.

Materials and methods

Toddalolactone metabolic stabilities were investigated by incubating toddalolactone (20 μM) with liver microsomes from humans, rabbits, mice, rats, dogs, minipigs, and monkeys for 0, 30, 60, and 90 min. The CYP isoforms involved in toddalolactone metabolism were characterized based on chemical inhibition studies and screening assays. The effects of toddalolactone (0, 10, and 50 µM) on CYP1A1 and CYP3A5 protein expression were investigated by immunoblotting. After injecting toddalolactone (10 mg/

SUBMITTER: Shan L 

PROVIDER: S-EPMC9367672 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature