The Estrogen Receptor α Cistrome in Human Endometrium and Epithelial Organoids.

ABSTRACT: Endometrial health is affected by molecular processes that underlie estrogen responses. We assessed estrogen regulation of endometrial function by integrating the estrogen receptor α (ESR1) cistromes and transcriptomes of endometrial biopsies taken from the proliferative and mid-secretory phases of the menstrual cycle together with hormonally stimulated endometrial epithelial organoids. The cycle stage-specific ESR1 binding sites were determined by chromatin immunoprecipitation and next-generation sequencing and then integrated with changes in gene expression from RNA sequencing data to infer candidate ESR1 targets in normal endometrium. Genes with ESR1 binding in whole endometrium were enriched for chromatin modification and regulation of cell proliferation. The distribution of ESR1 bindi

SUBMITTER: Hewitt SC 

PROVIDER: S-EPMC9368022 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature