Dataset Information

Overexpression of the Arabidopsis MACPF Protein AtMACP2 Promotes Pathogen Resistance by Activating SA Signaling.

ABSTRACT: Immune response in plants is tightly regulated by the coordination of the cell surface and intracellular receptors. In animals, the membrane attack complex/perforin-like (MACPF) protein superfamily creates oligomeric pore structures on the cell surface during pathogen infection. However, the function and molecular mechanism of MACPF proteins in plant pathogen responses remain largely unclear. In this study, we identified an Arabidopsis MACP2 and investigated the responsiveness of this protein during both bacterial and fungal pathogens. We suggest that MACP2 induces programmed cell death, bacterial pathogen resistance, and necrotrophic fungal pathogen sensitivity by activating the biosynthesis of tryptophan-derived indole glucosinolates and the salicylic acid signaling pathway dependent on the activity of enhanced disease susceptibility 1 (EDS1). Moreover, the response of MACP2 mRNA isoforms upon pathogen attack is differentially regulated by a posttranscriptional mechanism: alternative splicing. In comparison to previously reported MACPFs in Arabidopsis, MACP2 shares a redundant but nonoverlapping role in plant immunity. Thus, our findings provide novel insights and genetic tools for the MACPF family in maintaining SA accumulation in response to pathogens in Arabidopsis.

SUBMITTER: Zhang X

PROVIDER: S-EPMC9369274 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Overexpression of the <i>Arabidopsis</i> MACPF Protein AtMACP2 Promotes Pathogen Resistance by Activating SA Signaling.

Zhang Xue X Dai Yang-Shuo YS Wang Yu-Xin YX Su Ze-Zhuo ZZ Yu Lu-Jun LJ Zhang Zhen-Fei ZF Xiao Shi S Chen Qin-Fang QF

International journal of molecular sciences 20220807 15

Immune response in plants is tightly regulated by the coordination of the cell surface and intracellular receptors. In animals, the membrane attack complex/perforin-like (MACPF) protein superfamily creates oligomeric pore structures on the cell surface during pathogen infection. However, the function and molecular mechanism of MACPF proteins in plant pathogen responses remain largely unclear. In this study, we identified an Arabidopsis MACP2 and investigated the responsiveness of this protein du ...[more]

PMID: 35955922

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Dataset Information

Overexpression of the Arabidopsis MACPF Protein AtMACP2 Promotes Pathogen Resistance by Activating SA Signaling.

Publications

Overexpression of the <i>Arabidopsis</i> MACPF Protein AtMACP2 Promotes Pathogen Resistance by Activating SA Signaling.

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