Quantitative, in situ visualization of intracellular insulin vesicles in pancreatic beta cells.

ABSTRACT: Characterizing relationships between Zn²⁺, insulin, and insulin vesicles is of vital importance to the study of pancreatic beta cells. However, the precise content of Zn²⁺ and the specific location of insulin inside insulin vesicles are not clear, which hinders a thorough understanding of the insulin secretion process and diseases caused by blood sugar dysregulation. Here, we demonstrated the colocalization of Zn²⁺ and insulin in both single extracellular insulin vesicles and pancreatic beta cells by using an X-ray scanning coherent diffraction imaging (ptychography) technique. We also analyzed the elemental Zn²⁺ and Ca²⁺ contents of insulin vesicles using electron microscopy and energy dispersive spectroscopy (EDS) mapping. We found t

SUBMITTER: Guo A 

PROVIDER: S-EPMC9371705 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature