Efficient genome editing in wild strains of mice using the i-GONAD method.

ABSTRACT: Wild mouse strains have been used for many research studies, because of the high level of inter-strain genetic and phenotypic variations in them, in addition to the characteristic phenotype maintained from wild mice. However, since application of the current genetic engineering method on wild strains is not easy, there are limited studies that have attempted to apply gene modification techniques in wild strains. Recently, i-GONAD, a new method for genome editing that does not involve any ex vivo manipulation of unfertilized or fertilized eggs has been reported. We applied i-GONAD method for genome editing on a series of wild strains and showed that genome editing is efficiently possible using this method. We successfully made genetically engineered mice in seven out of the nine wild strain

SUBMITTER: Imai Y 

PROVIDER: S-EPMC9378668 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature