Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme.

ABSTRACT: Nature programs the structural folding of an enzyme that allows its on-demand biofunctionality; however, it is still a long-standing challenge to manually modulate an enzyme's conformation. Here, we design an exogenous hydrogen-bonded organic framework to modulate the conformation of cytochrome c, and hence allow non-native bioactivity for the enzyme. The rigid hydrogen-bonded organic framework, with net-arranged carboxylate inner cage, is in situ installed onto the native cytochrome c. The resultant hydrogen-bonded nano-biointerface changes the conformation to a previously not achieved catalase-like species within the reported cytochrome c-porous organic framework systems. In addition, the preserved hydrogen-bonded organic framework can stabilize the encapsulated enzyme and its channel-li

SUBMITTER: Chen G 

PROVIDER: S-EPMC9381776 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature