Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist.

ABSTRACT:

Background

 CLEC-2 is a platelet receptor with an important role in thromboinflammation but a minor role in hemostasis. Two endogenous ligands of CLEC-2 have been identified, the transmembrane protein podoplanin and iron-containing porphyrin hemin, which is formed following hemolysis from red blood cells. Other exogenous ligands such as rhodocytin have contributed to our understanding of the role of CLEC-2.

Objectives

 To identify novel CLEC-2 small-molecule ligands to aid therapeutic targeting of CLEC-2.

Methods

 ALPHA screen technology has been used for the development of a high-throughput screening (HTS) assay recapitulating the podoplanin-CLEC-2 interaction. Light transmission aggregometry was used to evaluate platelet aggregation. Immunoprecipitation and western

SUBMITTER: Moran LA 

PROVIDER: S-EPMC9393086 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature