The difference in CD4⁺ T cell immunity between high- and low-virulence Tembusu viruses is mainly related to residues 151 and 304 in the envelope protein.

ABSTRACT: Tembusu virus (TMUV) can result in a severe disease affecting domestic ducks. The role of T cells in protection from TMUV infection and the molecular basis of T cell-mediated protection against TMUV remain largely uncharacterized. Here, we used the high-virulence TMUV strain Y and the low-virulence TMUV strain PS to investigate the protective role for TMUV-specific CD4⁺ and CD8⁺ T cells. When tested in a 5-day-old Pekin duck model, Y and PS induced comparable levels of neutralizing antibody, whereas Y elicited significantly stronger cellular immune response relative to PS. Using a duck adoptive transfer model, we showed that both CD4⁺ and CD8⁺ T cells provided significant protection from TMUV-related disease, with CD8⁺ T cell conferrin

SUBMITTER: Meng R 

PROVIDER: S-EPMC9395619 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature