Delineating Mycobacterium abscessus population structure and transmission employing high-resolution core genome multilocus sequence typing.

ABSTRACT: Mycobacterium abscessus is an emerging multidrug-resistant non-tuberculous mycobacterium that causes a wide spectrum of infections and has caused several local outbreaks worldwide. To facilitate standardized prospective molecular surveillance, we established a novel core genome multilocus sequence typing (cgMLST) scheme. Whole genome sequencing data of 1991 isolates were employed to validate the scheme, re-analyze global population structure and set genetic distance thresholds for cluster detection and taxonomic identification. We confirmed and amended the nomenclature of the main dominant circulating clones and found that these also correlate well with traditional 7-loci MLST. Dominant circulating clones could be linked to a corresponding reference genome with less than 250 alleles while

SUBMITTER: Diricks M 

PROVIDER: S-EPMC9399081 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature