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Crustacean leg regeneration restores complex microanatomy and cell diversity.

ABSTRACT: Animals can regenerate complex organs, yet this process frequently results in imprecise replicas of the original structure. In the crustacean Parhyale, embryonic and regenerating legs differ in gene expression dynamics but produce apparently similar mature structures. We examine the fidelity of Parhyale leg regeneration using complementary approaches to investigate microanatomy, sensory function, cellular composition, and cell molecular profiles. We find that regeneration precisely replicates the complex microanatomy and spatial distribution of external sensory organs and restores their sensory function. Single-nuclei sequencing shows that regenerated and uninjured legs are indistinguishable in terms of cell-type composition and transcriptional profiles. This remarkable fidelity highlights the ability of organisms to achieve identical outcomes via distinct processes.

SUBMITTER: Almazan A

PROVIDER: S-EPMC9401613 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Crustacean leg regeneration restores complex microanatomy and cell diversity.

Almazán Alba A Çevrim Çağrı Ç Musser Jacob M JM Averof Michalis M Paris Mathilde M

Science advances 20220824 34

Animals can regenerate complex organs, yet this process frequently results in imprecise replicas of the original structure. In the crustacean <i>Parhyale</i>, embryonic and regenerating legs differ in gene expression dynamics but produce apparently similar mature structures. We examine the fidelity of <i>Parhyale</i> leg regeneration using complementary approaches to investigate microanatomy, sensory function, cellular composition, and cell molecular profiles. We find that regeneration precisely ...[more]

PMID: 36001670

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Crustacean leg regeneration restores complex microanatomy and cell diversity.

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Crustacean leg regeneration restores complex microanatomy and cell diversity.

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