A Mouse Holder for Awake Functional Imaging in Unanesthetized Mice: Applications in ³¹P Spectroscopy, Manganese-Enhanced Magnetic Resonance Imaging Studies, and Resting-State Functional Magnetic Resonance Imaging.

ABSTRACT: Anesthesia is often used in preclinical imaging studies that incorporate mouse or rat models. However, multiple reports indicate that anesthesia has significant physiological impacts. Thus, there has been great interest in performing imaging studies in awake, unanesthetized animals to obtain accurate results without the confounding physiological effects of anesthesia. Here, we describe a newly designed mouse holder that is interfaceable with existing MRI systems and enables awake in vivo mouse imaging. This holder significantly reduces head movement of the awake animal compared to previously designed holders and allows for the acquisition of improved anatomical images. In addition to applications in anatomical T₂-weighted magnetic resonance imaging (MRI), we also describe

SUBMITTER: Fadel LC 

PROVIDER: S-EPMC9406174 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature