Downstream Neighbor of Son Overexpression is Associated With Breast Cancer Progression and a Poor Prognosis.

ABSTRACT:

Purpose

The incidence rate of breast cancer (BC) has increased annually. Downstream neighbor of son (DONSON) critically affects cell cycle progression and maintains stable genomic properties; however, its relevant effects on BC growth and progression require in-depth investigation.

Methods

DONSON upregulation was validated in public databases. DONSON expression in matched BC and adjacent tissues and cell lines (MDA-MB-231, BT-549, and HS-578T) was determined using quantitative reverse transcription polymerase chain reaction. In vitro apoptosis, invasion, migration, and proliferation tests were performed to ascertain the functions of DONSON in BC cell lines. Then, using western blot analysis, the levels of DONSON downstream proteins were determined.

Results

Comp

SUBMITTER: Qi Y 

PROVIDER: S-EPMC9411031 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature