Evaluation of Normalization Approaches for Quantitative Analysis of Bile Acids in Human Feces.

ABSTRACT: Quantitative analysis of bile acids in human feces can potentially help to better understand the influence of the gut microbiome and diet on human health. Feces is a highly heterogeneous sample matrix, mainly consisting of water and indigestible solid material (as plant fibers) that show high inter-individual variability. To compare bile acid concentrations among different individuals, a reliable normalization approach is needed. Here, we compared the impact of three normalization approaches, namely sample wet weight, dry weight, and protein concentration, on the absolute concentrations of fecal bile acids. Bile acid concentrations were determined in 70 feces samples from healthy humans. Our data show that bile acid concentrations normalized by the three different approaches are substantia

SUBMITTER: Schott HF 

PROVIDER: S-EPMC9416035 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature