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Chiral 3D DNA origami structures for ordered heterologous arrays.

ABSTRACT: The DNA origami technique allows the facile design and production of three-dimensional shapes from single template strands of DNA. These can act as functional devices with multiple potential applications but are constrained by practical limitations on size. Multi-functionality could be achieved by connecting together distinct DNA origami modules in an ordered manner. Arraying of non-identical, three-dimensional DNA origamis in an ordered manner is challenging due for example, to a lack of compatible rotational symmetries. Here we show that we can design and build ordered DNA structures using non-identical 3D building blocks by using DNA origami snub-cubes in left-handed and right-handed forms. These can be modified such that one form only binds to the opposite-handed form allowing regular arrays wherein building blocks demonstrate alternating chirality.

SUBMITTER: Islam MS

PROVIDER: S-EPMC9418780 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Chiral 3D DNA origami structures for ordered heterologous arrays.

Islam Md Sirajul MS Wilkens Gerrit David GD Wolski Karol K Zapotoczny Szczepan S Heddle Jonathan Gardiner JG

Nanoscale advances 20210705 16

The DNA origami technique allows the facile design and production of three-dimensional shapes from single template strands of DNA. These can act as functional devices with multiple potential applications but are constrained by practical limitations on size. Multi-functionality could be achieved by connecting together distinct DNA origami modules in an ordered manner. Arraying of non-identical, three-dimensional DNA origamis in an ordered manner is challenging due for example, to a lack of compat ...[more]

PMID: 36134307

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