The first apicoplast tRNA thiouridylase plays a vital role in the growth of Toxoplasma gondii.

ABSTRACT: Toxoplasmosis caused by the protozoan Toxoplasma gondii is one of the most common parasitic diseases in humans and almost all warm-blooded animals. Lys, Glu, and Gln-specific tRNAs contain a super-modified 2-thiourea (s²U) derivatives at the position 34, which is essential for all living organisms by maintaining the structural stability and aminoacylation of tRNA, and the precision and efficiency of codon recognition during protein translation. However, the enzyme(s) involved in this modification in T. gondii remains elusive. In this report, three putative tRNA-specific 2-thiolation enzymes were identified, of which two were involved in the s²U34 modification of tRNA^Lys, tRNA^Glu, and tRNA^Gln. One was named TgMnmA,

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9420914 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature