SHOX2 refines the identification of human sinoatrial nodal cell population in the in vitro cardiac differentiation.

ABSTRACT:

Introduction

Dysfunction of the sinoatrial node (SAN) cells causes arrhythmias, and many patients require artificial cardiac pacemaker implantation. However, the mechanism of impaired SAN automaticity remains unknown, and the generation of human SAN cells in vitro may provide a platform for understanding the pathogenesis of SAN dysfunction. The short stature homeobox 2 (SHOX2) and hyperpolarization-activated cyclic nucleotide-gated cation channel 4 (HCN4) genes are specifically expressed in SAN cells and are important for SAN development and automaticity. In this study, we aimed to purify and characterize human SAN-like cells in vitro, using HCN4 and SHOX2 as SAN markers.

Methods

We developed an HCN4-EGFP/SHOX2-mCherry dual reporter cell line deri

SUBMITTER: Wakimizu T 

PROVIDER: S-EPMC9420958 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature