Transcriptomic Responses of Human Retinal Vascular Endothelial Cells to Inflammatory Cytokines.

ABSTRACT:

Purpose

Molecular profiling of human retinal endothelial cells provides opportunities to understand the roles of this cell population in maintenance of the blood-ocular barrier, and its involvements in diverse retinal vasculopathies. We aimed to generate a transcriptome of human retinal endothelial cells in the unstimulated state, and following treatment with inflammatory cytokines linked to cell dysfunction.

Methods

Endothelial cells were isolated from retinae of five human cadaveric donors, and treated for 60 minutes and 24 hours with interleukin-1β or tumor necrosis factor-α, or exposed to medium alone for the same intervals. Expression of intercellular adhesion molecule-1 was measured by RT-qPCR to confirm cytokine-induced activation of the cells. RNA was sequenced on th

SUBMITTER: Ryan FJ 

PROVIDER: S-EPMC9428361 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature