A pilot study to troubleshoot quality control metrics when assessing circulating miRNA expression data reproducibility across study sites.

ABSTRACT:

Background

Given the growing interest in using microRNAs (miRNAs) as biomarkers of early disease, establishment of robust protocols and platforms for miRNA quantification in biological fluids is critical.

Objective

The goal of this multi-center pilot study was to evaluate the reproducibility of NanoString nCounter™ technology when analyzing the abundance of miRNAs in plasma and cystic fluid from patients with pancreatic lesions.

Methods

Using sample triplicates analyzed across three study sites, we assessed potential sources of variability (RNA isolation, sample processing/ligation, hybridization, and lot-to-lot variability) that may contribute to suboptimal reproducibility of miRNA abundance when using nCounter™, and evaluated expression of positive and negative cont

SUBMITTER: Permuth JB 

PROVIDER: S-EPMC9428925 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature