Millisecond Time-Resolved Solid-State NMR Initiated by Rapid Inverse Temperature Jumps.

ABSTRACT: Elucidation of the detailed mechanisms by which biological macromolecules undergo major structural conversions, such as folding, complex formation, and self-assembly, is a central concern of biophysical chemistry that will benefit from new experimental methods. We describe a simple technique for initiating a structural conversion process by a rapid decrease in the temperature of a solution, i.e., a rapid inverse temperature jump. By pumping solutions through copper capillary tubes that are thermally anchored to heated and cooled blocks, solution temperatures can be switched from 95 to 30 °C (or lower) in about 0.8 ms. For time-resolved solid-state nuclear magnetic resonance (ssNMR), solutions can then be frozen rapidly by spraying into cold isopentane after a variable structural evolution

SUBMITTER: Wilson CB 

PROVIDER: S-EPMC9429955 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature