Integrated single-cell transcriptomic analyses reveal that GPNMB-high macrophages promote PN-MES transition and impede T cell activation in GBM.

ABSTRACT:

Background

Glioblastoma (GBM) is the most aggressive type of primary brain tumor and is often resistant to current therapies. Tumor microenvironment-centered therapies may unleash new hope for GBM treatment. Therefore, an in-depth understanding of tumor-stroma communication is urgently needed to identify promising therapeutic targets.

Methods

We systematically analyzed GBM single-cell RNA sequencing (scRNA-seq), bulk RNA-seq and spatial scRNA-seq data from various human and mice studies to characterize the network within the microenvironment. Moreover, we applied ex vivo co-culture system, flow cytometry analysis and immunofluorescent staining to validate our findings.

Findings

Our integrative analyses revealed that highly heterogeneous GBM tumor cells can be classifi

SUBMITTER: Xiong A 

PROVIDER: S-EPMC9437813 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature