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Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.

ABSTRACT: Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10³ TCID₅₀/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.

SUBMITTER: Te N

PROVIDER: S-EPMC9438371 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.

Te Nigeer N Rodon Jordi J Creve Rhea R Pérez Mónica M Segalés Joaquim J Vergara-Alert Júlia J Bensaid Albert A

Veterinary research 20220902 1

Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10<sup>3</sup> TCID<sub>50</sub>/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in res ...[more]

PMID: 36056449

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Transcriptional responses of Middle East respiratory syndrome coronavirus (MERS-CoV) in marmosets

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Project description: Not available

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