Induction of the BRAFV600E Mutation in Thyroid Cells Leads to Frequent Hypermethylation.

ABSTRACT:

Objectives

The BRAFV600E mutation is a major driver mutation in papillary thyroid cancer. The aim of this study was to elucidate the correlation between DNA methylation and gene expression changes induced by the BRAFV600E mutation in thyroid cells.

Methods

We used Nthy/BRAF cell lines generated by transfection of Nthy/ori cells with the wild-type BRAF gene (Nthy/WT cells) and the V600E mutant-type BRAF gene (Nthy/V600E cells). We performed gene expression microarray and DNA methylation array analyses for Nthy/WT and Nthy/V600E cells. Two types of array data were integrated to identify inverse correlations between methylation and gene expression. The results were verified in silico using data from The Cancer Genome Atlas (TCGA) and in vivo through pyrosequencing and quantitat

SUBMITTER: Yi JW 

PROVIDER: S-EPMC9441509 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature