Effect of lactoferrin on ram sperm motility after cryopreservation.

ABSTRACT:

Objective

The objective of this study was to analyse the differentially abundant proteins caused by freeze-thawing of ram sperm and explore candidate proteins of interest for their ability to improve ram sperm cryopreservation outcomes in vitro.

Methods

Sperm were from three mature Dorper. Fresh and frozen sperm proteins were extracted, and the differentially abundant proteins were analysed by mass spectrometry. Among these proteins, lactoferrin (LTF) was selected to be added before cryopreservation. Next, sperm samples were diluted in Tris extender, with the addition of 0, 10, 100, 500, and 1,000 μg/mL of LTF. After thawing, sperm quality was evaluated by motility, plasma membrane integrity, mitochondrial activity and reactive oxygen species (ROS).

Results

Cryopreser

SUBMITTER: Su J 

PROVIDER: S-EPMC9449401 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature