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An optimized mouse model of Staphylococcus aureus infected diabetic ulcers.

ABSTRACT:

Objective

Diabetic foot infection (DFI) represents a major healthcare burden, for which treatment is challenging owing to the pathophysiological alterations intrinsic to diabetes and the alarming increase of antimicrobial resistance. Novel therapies targeting DFI are therefore a pressing research need for which proper models of disease are required.

Results

Here, we present an optimized diabetic mouse model of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds, that resemble key features of DFI, such as pathogen invasion through wound bed and surrounding tissue, necrosis, persistent inflammation and impaired wound healing. Thus, in a time-efficient manner and using simple techniques, this model represents a suitable approach for studying emerging therapies targeting DFI caused by MRSA.

SUBMITTER: Mendes AI

PROVIDER: S-EPMC9450231 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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An optimized mouse model of Staphylococcus aureus infected diabetic ulcers.

Mendes Ana Isabel AI Peixoto Maria João MJ Marques Alexandra Pinto AP Pedrosa Jorge J Fraga Alexandra Gabriel AG

BMC research notes 20220907 1

<h4>Objective</h4>Diabetic foot infection (DFI) represents a major healthcare burden, for which treatment is challenging owing to the pathophysiological alterations intrinsic to diabetes and the alarming increase of antimicrobial resistance. Novel therapies targeting DFI are therefore a pressing research need for which proper models of disease are required.<h4>Results</h4>Here, we present an optimized diabetic mouse model of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds, tha ...[more]

PMID: 36071445

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