Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.

ABSTRACT: Cottonseed meal (CSM) is a good source of dietary proteins but is unsuitable for human consumption due to its gossypol content. To unlock its potential, we developed a protein extraction process with a gossypol removal treatment to generate CSM protein isolate (CSMPI) with ultra-low gossypol content. This process successfully reduced the free and total gossypol content to 4.8 ppm and 147.2 ppm, respectively, far below the US FDA limit. In addition, the functional characterisation of CSMPI revealed a better oil absorption capacity and water solubility than pea protein isolate. Proteome profiling showed that the treatment improved protein identification, while SDS-PAGE analysis indicated that the treatment did not induce protein degradation. Amino acid analysis revealed that post-treated CSM

SUBMITTER: Tan CF 

PROVIDER: S-EPMC9455987 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature