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Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by Toxoplasma gondii.

ABSTRACT:

Background

Toxoplasma gondii, an intracellular protozoan parasite, exists in the host brain as cysts, which can result in Toxoplasmic Encephalitis (TE) and neurological diseases. However, few studies have been conducted on TE, particularly on how to prevent it. Previous proteomics studies have showed that the expression of C3 in rat brains was up-regulated after T. gondii infection.

Methods

In this study, we used T. gondii to infect mice and bEnd 3 cells to confirm the relation between T. gondii and the expression of C3. BEnd3 cells membrane proteins which directly interacted with C3a were screened by pull down. Finally, animal behavior experiments were conducted to compare the differences in the inhibitory ability of TE by four chemotherapeutic compounds (SB290157, CVF, NSC23766, and Anxa1).

Results

All chemotherapeutic compounds in this study can inhibit TE and cognitive behavior in the host. However, Anxa 1 is the most suitable material to inhibit mice TE.

Conclusion

T. gondii infection promotes TE by promoting host C3 production. Anxa1 was selected as the most appropriate material to prevent TE among four chemotherapeutic compounds closely related to C3.

SUBMITTER: Yan Z

PROVIDER: S-EPMC9457825 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by <i>Toxoplasma gondii</i>.

Yan Zijing Z Yuan Hao H Wang Junjie J Yang Zipeng Z Zhang Pian P Mahmmod Yasser S YS Wang Xiaohu X Liu Tanghui T Song Yining Y Ren Zhaowen Z Zhang Xiu-Xiang XX Yuan Zi-Guo ZG

Molecules (Basel, Switzerland) 20220830 17

<h4>Background</h4><i>Toxoplasma gondii</i>, an intracellular protozoan parasite, exists in the host brain as cysts, which can result in Toxoplasmic Encephalitis (TE) and neurological diseases. However, few studies have been conducted on TE, particularly on how to prevent it. Previous proteomics studies have showed that the expression of C3 in rat brains was up-regulated after <i>T. gondii</i> infection.<h4>Methods</h4>In this study, we used <i>T. gondii</i> to infect mice and bEnd 3 cells to co ...[more]

PMID: 36080339

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