Performance evaluation of presepsin using a Sysmex HISCL-5000 analyzer and determination of reference interval.

ABSTRACT:

Background

Analytical evaluation of newly developed presepsin by a Sysmex HISCL-5000 (Sysmex, Japan) automated immune analyzer was performed.

Methods

For evaluation, sepsis patient samples were collected before treatment in an emergency department. Precision, linearity, limit of blank/limit of detection, method comparisons, and reference intervals were evaluated. Method comparisons were performed using a PATHFAST immune analyzer (LSI Medience Corporation, Japan).

Results

Precision using a 20x2x2 protocol for low (306 pg/mL) and high (1031 pg/mL) levels resulted in within-laboratory standard deviation (95% confidence interval [CI]) and coefficient of variation (CV) %, which were as follows: 15.3 (13.1-18.7), 5.5% and 47.7, (40.5-58.1), 6.4%, respectively. Linearity usi

SUBMITTER: Kang T 

PROVIDER: S-EPMC9459287 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature