Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.

ABSTRACT:

Significance

Optical imaging of responses in fluorescently labeled neurons has progressed significantly in recent years. However, there is still a need to monitor neural activities at divergent spatial scales and at depths beyond the optical diffusion limit.

Aim

To meet these needs, we aim to develop multiscale photoacoustic tomography (PAT) to image neural activities across spatial scales with a genetically encoded calcium indicator GCaMP.

Approach

First, using photoacoustic microscopy, we show that depth-resolved GCaMP signals can be monitored in vivo from a fly brain in response to odor stimulation without depth scanning and even with the cuticle intact. In vivo monitoring of GCaMP signals was also demonstrated in mouse brains. Next, using photoacoustic computed to

SUBMITTER: Zhang R 

PROVIDER: S-EPMC9463545 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature