Roles of microglia/macrophage and antibody in cell sheet transplantation in the central nervous system.

ABSTRACT:

Background

We previously established a human mesenchymal stem cell (MSC) line that was modified to express trophic factors. Transplanting a cell sheet produced from this line in an amyotrophic lateral sclerosis mouse model showed a beneficial trend for mouse life spans. However, the sheet survived for less than 14 days, and numerous microglia and macrophages were observed within and adjacent to the sheet. Here, we examined the roles of microglia and macrophages as well as acquired antibodies in cell sheet transplantation.

Methods

We observed the effects of several MSC lines on macrophages in vitro, that is, phenotype polarization (M1 or M2) and migration. We then investigated how phenotypic polarization affected MSC survival using antibody-dependent cellular cytotoxicity (AD

SUBMITTER: Honda N 

PROVIDER: S-EPMC9465875 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature