Toward high-throughput oligomer detection and classification for early-stage aggregation of amyloidogenic protein.

ABSTRACT: Aggregation kinetics of proteins and peptides have been studied extensively due to their significance in many human diseases, including neurodegenerative disorders, and the roles they play in some key physiological processes. However, most of these studies have been performed as bulk measurements using Thioflavin T or other fluorescence turn-on reagents as indicators of fibrillization. Such techniques are highly successful in making inferences about the nucleation and growth mechanism of fibrils, yet cannot directly measure assembly reactions at low protein concentrations which is the case for amyloid-β (Aβ) peptide under physiological conditions. In particular, the evolution from monomer to low-order oligomer in early stages of aggregation cannot be detected. Single-molecule methods allow

SUBMITTER: Tahirbegi B 

PROVIDER: S-EPMC9468268 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature